2017-05-24 13:35:582025-05-25 14:30:31
description
extracellular neutral protease B
extracellular neutral protease B, required for the function of the [[protein|YitM]] toxin
locus
BSU11100
BSU_11100
geneLength
1614
1617
function
degradation of proteins
protection of B. subtilis biofilms against competitors
ec
outlinks
bsu
BSU11100
BSU_11100
Gene
Coordinates
1,186,037 → 1,187,653
1,186,037 1,187,653
The protein
Catalyzed reaction/ biological activity
Similar, but not identical, to that of thermolysin (according to Swiss-Prot)
The protein
Protein family
peptidase M4 family (according to Swiss-Prot)
peptidase M4 family (with [[protein|NprE]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|NprE]]
[[this]]
Biological materials
Mutant
BKE11100 (''ΔnprB::erm trpC2''), available at BGSC
KO7 (''ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr''), available as BGSC 1A1133
KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
BKE11100 ([[gene|nprB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACACCACATCCTTCCT, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
BKK11100 ([[gene|nprB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACACCACATCCTTCCT, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
References
Original publications
The protein
Structure
[PDB|5A3Y] (thermolysin, 44% identity) [pubmed|26527148]